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What that does is it takes the sequence you enter and “pretends it’s a ribosome” – it (in make-believe-land) translates all 6 possible open reading frames (ORFs).
Ggu amino acid software#
You can use online software like Expasy translate to do this (and there are a bunch of other tools if you just Google it). If you have a nucleic acid sequence (DNA or RNA form) you might want to know what the corresponding amino acid sequence is – so you want to “translate” it. So you have gene (DNA) gets transcribed -> pre-mRNA gets processed (introns spliced out, cap and tail added) -> mature mRNA gets translated by ribosomes -> protein When a cell wants to make a protein, it makes a messenger RNA (mRNA) copy of one strand of the DNA and that is used by ribosomes to make the encoded protein in a process called translation. In this genomic DNA, there are parts that have protein-making instructions (coding parts aka exons) and parts which “just” have regulatory instructions (non-coding parts – introns (parts between exons that get removed during mRNA processing) and intergenic regions (parts between genes)).
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And the instructions for them are kept in the form of double-stranded DNA (dsDNA) as part of long chromosomes. The basic gist is that proteins are like molecular workers, made up of a string of amino acid letters. Today’s post is more technical, so I’m going to assume that if you care enough to read it, you know a bit about nucleic acids (DNA and RNA) already (and will provide links to relevant posts if you want to review). So put on your reading frames and let’s go! Pretend-playing with nucleic acids: using free software tools to manipulate DNA and RNA sequences to get them in the form you need and figure out what proteins they might code for… A tech tip post on complementing, reverse-complementing, translating, and more! Basically, a post trying to explain some of the nucleic acid sequence terminology that might trip you up and providing some tips and tools to help.